Pyrogallol hydroxytransferase

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Pyrogallol hydroxytransferase
Identifiers
EC no.5.4.4.9
CAS no.125978-84-9
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MetaCycmetabolic pathway
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Pyrogallol hydroxytransferase (EC 5.4.4.9) is an enzyme that catalyzes a chemical reaction that converts pyrogallol to phloroglucinol by formation of the intermediate 1,2,3,5-tetrahydroxybenzene.[1]

 
 
 
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The enzyme isolated from the bacterium Pelobacter acidigallici contains molybdenum but the hydroxyl group which is initially added to pyrogallol does not come from water. Instead, some sulfoxides and aromatic N-oxides can provide these groups.[2][3]

Nomenclature

This enzyme belongs to the family of oxidoreductases. The systematic name of this enzyme class is 1,2,3,5-tetrahydroxybenzene:1,2,3-trihydroxybenzene hydroxytransferase. Other names in common use include 1,2,3,5-tetrahydroxybenzene hydroxyltransferase, 1,2,3,5-tetrahydroxybenzene:pyrogallol transhydroxylase, 1,2,3,5-tetrahydroxybenzene-pyrogallol hydroxyltransferase (transhydroxylase), pyrogallol hydroxyltransferase, 1,2,3,5-tetrahydroxybenzene:1,2,3-trihydroxybenzene hydroxyltransferase.[4]

References

  1. Brune A, Schink B (February 1990). "Pyrogallol-to-phloroglucinol conversion and other hydroxyl-transfer reactions catalyzed by cell extracts of Pelobacter acidigallici". Journal of Bacteriology. 172 (2): 1070–1076. doi:10.1128/jb.172.2.1070-1076.1990. PMC 208538. PMID 2298693.
  2. Reichenbecher W, Brune A, Schink B (February 1994). "Transhydroxylase of Pelobacter acidigallici: a molybdoenzyme catalyzing the conversion of pyrogallol to phloroglucinol". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1204 (2): 217–224. doi:10.1016/0167-4838(94)90011-6. PMID 8142462.
  3. Reichenbecher W, Schink B (March 1999). "Towards the reaction mechanism of pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1430 (2): 245–253. doi:10.1016/s0167-4838(99)00004-7. PMID 10082952.
  4. Enzyme 5.4.4.9 at KEGG Pathway Database.